Main Activities

·    Production of PDMS cavities on electron microscopy grids

·    Cryo-electron tomography specimen preparation of cells cultured in PDMS cavities

·    Cryo-electron tomography data acquisition

·    Cryo-electron tomography data processing, including pre-processing, tomogram reconstruction, ultrastructural analysis and structure determination by subtomogram averaging

·    Note-keeping, reporting and discussing results within the team

·    Direct collaboration with PhD students in the team and in collaborating team

Associated Activities

·    Giving scientific presentations at the institute and at international conferences

·    Writing scientific manuscripts

·    Participation in outreach events

·    Supervision of intern students

Specific Requirements or Constraints

·    Several days of business trips per year might be required

·    Handling of electron microscopy grids made from gold and PDMS microfabrication require high dexterity and precision

·    The project contains a large portion of data processing which is equivalent to long bouts of sedentary work

Skills/Qualifications

·    PDMS micorfabrication

·    Mammalian cell culture

·    Cryo-EM/ET specimen preparation via high-pressure freezing

·    Image processing of cryo-EM/ET data

·    Working in Linux environments, both, local and on high-performance clusters

·    Scripting, preferentially in Python

·    Good communication skills in English both during scientific meetings and within the team